NUCLEIC dotMETRIC™
For double stranded DNA , use undiluted Nucleic Acid Dilution Buffer .
For RNA or oligos , dilute the Nucleic Acid Dilution Buffer 100 fold .
i . e . Use 1µl Nucleic Acid Dilution Buffer in 99µl ultrapure water .
Dilute samples at least 1 : 1 with the appropriate Dilution Buffer .
Open the NUCLEIC Test Strip box , remove a strip , and peel away the protective sheets using the forceps provided .
Attach the white NUCLEIC Strip to the application board with the side magnets .
Apply 1‐5µl of the diluted samples to the Test Strip using one of these two methods ( or see the Guidelines for using G‐Biosciences’ Spot Application Device ) :
Free‐Drop Method Of Application Squeeze the pipette plunger until a drop is formed at the tip of the pipette tip .
Lower the drop until it touches the Test Strip .
The nucleic acid solution drop will immediately spread on the Test Strip .
dotMETRIC™ Application Method .
Apply sample by point of contact capillary action .
Use pipette tips with an outside bore diameterof 0.6‐0.7 mm ( Cat . # 786‐64 ) .
Keep the pipette tip straight up and allow the capillary action of the strip to draw the sample from the pipette tip ( see figure below ) .
Do not use the plunger to force the solution out .
NOTE : Accurately pipetting 1µl requires skill and caution .
A deviation of up to ± 20 % could be due simply to pipetting errors .
In addition , applying more than > 1 µl or multiple spots are sometimes slow & tedious .
Samples do not need to dry before adding NUCLEIC Dye .
Place the NUCLEIC strip in a developing tray ( Cat . # 786‐024 ) or other small dish .
Apply 0.5ml NUCLEIC Dye directly to the strip .
Incubate at room temperature for 1 minute without shaking or rocking .
Discard the NUCLEIC Dye and rinse the strip for 10 seconds in 50ml ultra pure water .
Discard the rinse and wash with 50ml ultra pure water for 30‐60 seconds or until the blue background disappears .
Remove the strip from the second wash as soon as the blue background disappears .
For Free‐Drop Method : Compare the color of the test sample spot with the dotMETRIC™ Standard provided .
For dotMETRIC Application : Use the NUCLEIC dotMETRIC™ scale to determine the concentration of the diluted sample .
If a dot is not symmetrical measure both the widest and narrowest points of the dot and average .
