SpinOUT™ : Protein Desalting
Equilibrate the column with the buffer of choice by applying at least 5 column volumes of buffer in batches of 1 - 2 column volumes .
Centrifuge the column at 1,000g for 2 minutes to remove the buffer after each application .
Place the column in a new collection tube and remove the cap .
Slowly , apply the protein solution to the center of the SpinOUT™ resin .
Centrifuge the column at 1,000g for the indicated times in the table below to collect the desalted protein solution .
Discard the column .
Mark one side of the column and ensure in all centrifugations the mark is facing outwards during centrifugation .
Prepare the SpinOUT™ column by centrifuging the SpinOUT™ columns at 1,000g for 1 minute to compact the resin .
Remove the top and then bottom caps .
Place into an appropriate collection tube .
Centrifuge the column at 1,000g for 2 minutes to remove the storage buffer .
