Zymoclean™ Gel DNA Recovery Kit
Excise the DNA fragment from the agarose gel using a razor blade , scalpel or other device and transfer it into a 15 ml microcentrifuge tube .
Add 3 volumes of ADB to each volume of agarose excised from the gel .
Incubate at 37 - 55 °C for 5 - 10 minutes until the gel slice is completely dissolved .
Transfer the melted agarose solution to a Zymo - Spin™ Column in a Collection Tube .
Centrifuge for 30 - 60 seconds .
Discard the flow - through .
Wash # 1 : Add 200 µl of DNA Wash Buffer to the column .
Wash # 1 : Centrifuge for 30 seconds .
Discard the flow - through .
Wash # 2 : Add 200 µl of DNA Wash Buffer to the column .
Wash # 2 : Centrifuge for 30 seconds .
Discard the flow - through .
Add ≥ 6 µl DNA Elution Buffer or water directly to the column matrix .
Place column into a 1.5 ml tube and centrifuge for 30 - 60 seconds to elute DNA .
