PCR with Taq DNA Polymerase ( M0273 )
Set up the following reaction on ice .
Gently mix the reaction .
Collect all liquid to the bottom of the tube by a quick spin if necessary and overlay the sample with mineral oil if using a PCR machine without a heated lid .
Transfer PCR tubes from ice to a PCR machine with the block preheated to 95°C and begin thermocycling .
