How to make a 1M DTT Stock Solution
Weigh 1.54 g of Dithiothreitol ( DTT ) .
Add 10 ml of sterile dH2O .
Dissolve completely .
Prewet a 0.2 µm syringe filter by drawing through 5 - 10 ml of sterile H2O and discard water .
Sterilize DTT Stock through the prepared 0.2 µm syringe filter .
Aliquot into 2 ml tubes and store at - 20°C .
Stocks may be kept for 1 year .
