Cultivation / Spore Production for Alternaria Solani
Inoculate Mycelium with spores from the stock plate of fresh SNA plates .
Make sure to inoculate only a small amount right in the middle of the plate to allow even and equal spread and growth throughout the plate .
Store plate in a growth chamber with 12 / 12 h darkness / blacklight .
Optimal temperature is 22°C .
Optimal humidity 60 - 70 % .
Allow to grow for 3 weeks .
Harvest spores adding some 0.0 - 0.5 % tween solution to the plate and scraping gently with a coverslip .
Collect all liquid with spores Filter through four layers of mesh ( e . g . cheese cloth ) to remove mycelial debris .
