Colony PCR Protocol
Ligated DNA is transformed into cells .
Plate transformed cells on selection plate .
Go home , wait 13 hours .
Pick a well separated single colony from selection plate via yellow pipette tips .
Move said colony to 96 well microtittle plate .
Place individual colony into a single well containing 50 µl sterile distilled water .
Repeat for as many colonies as desired .
Make up PCR mix .
Use multichannel pipette to add 7.5 µl PCR mix to a 96 well PCR plate .
Add 2.5 µl colony solution .
Run PCR reaction .
Add 4 µl loading dye once PCR run is complete .
Load ( PCR reaction mix + dye ) into gel and run .
Complete run takes about 5 hours .
