DNA Extraction of Cesium Chloride - Purified Viruses using Wizard Prep Columns
Prepare 2 labeled 1.5ml microfuge tubes per sample and make sure resin is at room temperature .
Shake resin vigorously to resuspend particles .
Mix 1ml DNA Purification Resin with 0.5ml CsCl sample .
Attach minicolumn to bottom of 3ml or 5ml sterile syringe that has had plunger removed .
Add resin with sample to the syringe and push through the solution .
Remove minicolumn from the syringe and pull out plunger .
Reattach minicolumn to the syringe and 2ml of 80 % isopropanol to the syringe .
Using the plunger push through the isopropanol to wash the resin .
Remove minicolumn from syringe and place in a sterile 1.5ml centrifuge tube .
Centrifuge 10,000 g for 2min to remove any residual liquid .
Place minicolumn in new sterile 1.5ml centrifuge tube .
Add 100μl 80°C TE buffer to top of minicolumn .
Place tube lid over top of column and vortex gently for 10 seconds .
Wait 1 minute .
Centrifuge at 10,000 g for 30 sec to elute DNA .
Quantify DNA by Quant - iT PicoGreen dsDNA assay kit .
