RNAlater Recipe
Prepare 0.5 M EDTA .
Prepare 1 M Sodium citrate .
In a 2 or 3 L flask mix 1,400 mL MilliQ water with 60 mL 0.5M EDTA and 37.5 mL 1M sodium citrate with stirring .
While continuing to stir the solution , add 1.05 kg ( = 1,050 g ) Ammonium sulfate in 100 g amounts .
Stir ~ 30 min on hot plate set to low .
Allow solution to cool at room temperature for at least 30min , but overnight on bench is best .
Filter solution through 0.2 μm filter to remove particles .
Store at room temp or at 4°C .
