Plant Protoplast Encapsulation .
Gently load plant protoplast suspensions into hamilton gas - tight syringe .
Load 2.5 % wt % Pico - Surf ( TM ) 1in Novec 7500 into hamilton gas - tight syringe .
Place the microfluidic device to the stage of bright - field inverted microscope which connects with a high - speed camera .
Connect the syringes to the corresponding inlets of the microfluidic chip via PTFE microtubing .
Load onto Harvard Apparatus , PHD 2000 syringe pump and start infusing .
Record the encapsulation videos with the fast camera ( PHANTOM MIROEX4 ) using the manufacturer's software .
Image the plant protoplasts in droplets with Andor iXon Ultra 897 camera for fluorescence and bright field images , controlled with the Labview software .
