Double SPRI for Second Generation Sequencing
Add 65 µl SPRI Ampure beads to 50µl of sample.
Mix and incubate for 20 minutes.
Separate on magnet (6 min.)
Transfer all supernatant (about 115 µl) into a new well (off magnet).
Add 100 µl of SPRI beads.
Mix and incubate for 15 minutes.
Separate on magnet (6min), discard supernatant.
Wash beads with 60 µl of 70% EtOH.
Move well off magnet.
Dry beads of EtOH (~10min).
Add 40 µl EB.
Mix and incubate for 3 minutes.
Separate on magnet and transfer eluted product into destination plate
