ASP12A Recipe for culturing Aureococcus anophagefferens
Prepare Trace Metal Solutions I - III , Fe - EDTA Sodium Salt Solution , and Vitamin Solution .
Add constituents to 750 mL MilliQ water , shake to dissolve .
Add MilliQ water to 1.0 L .
Filter sterilize .
Trace Metal Solutions and Fe - EDTA Sodium Salt Solution are stored at 4ºC in 15 - 50 mL aliquots , while the Vitamin solution is stored at - 20ºC in 5 - 10 mL aliquots .
Add anhydrous salts , hydrous salts , macronutrients and Tris Base to 75 % desired MilliQ water while stirring to dissolve .
Add MilliQ water to final volume , autoclave .
Once cooled , add in 1mL of the Trace Metal Solutions , Fe - EDTA Sodium Salt Solution , and Vitamin Solution per 1L of media made .
