Obtaining pure cyanophage stocks ( plaque purification ) .
Make a dilution series of the lysate ( assume 104 to 105 PFU per mL in the plaque lysate ) .
Repeat the plaque purification procedure 2 more times to ensure that the cyanophage isolated is clonal .
Repeat the plaque purification procedure again .
Finally , prepare a primary cyanophage stock using lysate from the final purification via method A " Liquid Amplification " OR method B " Plate Amplification " Method A : liquid amplification Method B : plate amplification Titer the final stock via plaque assay .
Cyanophage stocks stored at 4°C in the dark are stable for at least a year .
