DNA Extraction Protocol
Create mixture of CsCl , Proteinase K , and SDS .
Mix and incubate 1 hour at 56°C .
Cool to room temperature Add an equal volume of phenol and invert several times .
Spin 3000g ( 6000 rpm on microfuge ) , 5 minutes , room temperature .
Carefully transfer the supernatant with a wide - bore pipette to a fresh tube .
Add an equal volume of phenol : choroform ( 1 : 1 ) , invert .
Spin again , 3000g ( 6000 rpm on microfuge ) , 5 minutes , room temperature .
Transfer the supernatant , as done before .
Add an equal volume of chloroform and invert .
Spin again , 3000g ( 6000 rpm on microfuge ) , 5 minutes , room temperature .
Transfer the supernatant , as done before .
