Picking colonies
Number and annotate ( color / size ) 95 isolated colonies on SPOT 0m 10ml plates .
In 2ml deep 96 well plates add 1ml of Zobell broth to all wells using 8 channel pipet Using autoclaved / sterile 200ul pipet tips ( full box ) pick all 95 colonies .
Use pipet tip to pick up an entire isolated colony and place pipet tip into well .
Pick colonies 1 - 95 in column 1 A through H , column 2 1 through H and so on .
In well 96 ( 12 H ) place the final unused pipet tip as a Negative .
After all 96 wells have pipet tips in them , using a 12 channel pipet , attach each row of tips and pipet up and down at least 3 times to dislodge colony .
Remove tips after each row .
Place pore film over plate and seal well .
Label plate Place on shaker for at least 48 hours to grow
