One - Step Transformation of Yeast
Add 90 uL of 50 % Polyethylene Glycol 3350 to a 1.5 mL microcentrifuge tube .
Add 10 uL of 0.2 N Lithium Acetate to the microcentrifuge tube .
Pellet yeast from 1 mL liquid culture or take a toothpick full of freshly grown yeast on a plate and resuspend in the solution .
Add 1 ug of DNA and briefly vortex .
Incubate at 45°C for 30 minutes to 1 hour , vortexing briefly every 10 minutes .
Add 1 mL water to the tube and pipette to mix , then centrifuge at 14,000 RPM for 15 seconds .
Remove the supernatant and resuspend the pellet in 200 uL water , then spread on selective plates and incubate at 30°C for 3 - 6 days .
