Gibson Assembly® Protocol ( E5510 )
Incubate samples in a thermocycler at 50°C for 15 minutes when 2 or 3 fragments are being assembled or 60 minutes when 4 - 6 fragments are being assembled .
Store samples on ice or at –20°C for subsequent transformation .
Transform NEB 5 - alpha Competent E . coli cells ( provided with the kit ) with 2 μl of the assembly reaction , following the transformation protocol .
