How to Make a 0.5M TCEP Stock Solution
Weigh 5.73 g of TCEP .
Add 35 ml of cold molecular biology grade water to the vial , and dissolve the TCEP .
This resulting solution is very acidic , with an approximate pH of 2.5 .
Bring the solution to pH 7.0 with 10 N NaOH or 10 N KOH .
Bring the resulting solution to 40 ml with molecular biology grade water .
Aliquot into 1 ml volumes in freezer tubes and store at - 20°C .
