Preparing AQUIL Media
Nutrient Stock Add 1.42g Sodium Silicate to 50ml of UPW in a 50ml vial .
Add 0.43g Sodium Nitrate to 50ml of UPW in a 50ml vial .
Add 0.069g Sodium dihydrogen Phosphate to 50ml of UPW in a 50ml vial .
Vitamin Stock Add 0.037g of Vitamin B12 to 50ml of UPW in a 50ml vial .
Add 0.0061g of Biotin to 50ml of UPW in a 50ml vial .
Add 1.5g Thiamine to 50ml of UPW in a 50ml vial .
EDTA Stock Add all of the following dry chemicals into one clean 50ml vial in order to make EDTA stock .
Nutrient and vitamin stocks should be chelexed and filter - sterilized before use .
EDTA ( metals ) stock should be filter - sterilized .
To make AQUIL , add the following reagents to 1L of chelexed , microwave - sterilized ASW , or 1L microwave sterilized surface seawater .
Add 1ml EDTA stock , 1ml sodium silicate , 1ml sodium nitrate , 1ml sodium dihydrogen phosphate , 100ul vitamin B12 , 100ul biotin and 100ul thiamine stock .
Invert bottle twice for mixing .
