A Typical DNase I Reaction ( M0303 )
Resuspend 10 µg RNA in 1X DNase I Reaction Buffer to a final volume of 100 µl .
Add 2 units of DNase I Mix thoroughly .
Incubate at 37°C for 10 minutes .
Add 1 µl of 0.5 M EDTA ( to a final concentration of 5 mM ) .
Heat inactivate at 75°C for 10 minutes .
