Glycerol Stock
Add 600 uL of your inoculated liquid culture to a 2 mL screw top tube .
Add 200 uL autoclaved water to the screw top tube .
Add 200 uL glycerol to the screw top tube .
Screw the top on the tube and mix by inversion several times so no layers are present in the mixture .
Place mixed screw top tube into a - 80 C Freezer .
