Blunting Protocol ( M0203 )
Dissolve DNA in any 1X NEBuffer or T4 DNA Ligase Reaction Buffer .
Supplement with 100 μM of each dNTP .
Add 1 unit of T4 DNA Polymerase per microgram DNA .
Incubate 15 minutes at 12°C .
Stop reaction by adding EDTA to a final concentration of 10 mM .
Heat for 20 minutes at 75°C ( see references 1,2 ) .
