Cesium Chloride Dialysis for Viruses
Prepare MTN100 with 5x NaCl .
Prepare MTN100 with 3x NaCl .
Prepare MTN100 with 1x NaCl .
Bring volume to 1L with Milli - Q water .
Use Slide - A - Lyzer dialysis casettes ( Pierce # 66425 or # 87730 ; 10K MWCO , 0.5 - 3.0ml ) and follow the manufacturer’s directions for loading and dialysis of cassettes .
Step dialyze for ~ 30 min with the 5x NaCl MTN buffer .
Step dialyze for ~ 30 min with the 3x NaCl MTN buffer .
Step dialyze for ~ 30 min with the two changes of the 1x NaCl MTN , using at least 300 volumes of buffer to sample .
Stir gently at room temperature Remove the sample according to manufacturer’s directions Transfer to sterile , low bind tubes .
Store dialyzed particles at 4°C .
