Nile Red Staining of Drosophila Larval Tissues
Dissect tissues in ice cold PBS , keeping samples on ice until required sample size is obtained .
Fix tissues in 4 % PFA for 20 minutes .
Wash tissues 3x in PBS .
Stain samples in Nile Red at 0.5 ug / mL diluted in PBS for 1 hour .
Wash tissues 3x in PBS .
Carefully replace the PBS with mounting medium before transferring samples to slides and imaging .
Image immediately , or temporarily store slides at 4°C .
