CB - X™ Protein Assay for simple aqueous samples
Chill CB - X™ at - 20°C .
Prior to use , mix CB - X™ Assay Dye by gently inverting the bottle several times .
DO NOT SHAKE THE BOTTLE .
Dilute the supplied protein standards in the same buffer as your protein samples .
We recommend duplicates of 5 - 6 dilutions within the range of 0.1 - 1.0mg / ml .
Transfer 50µl diluted protein standards and test samples to the assay tubes .
Set up a blank containing 50µl of the buffer used to generate the standards .
Add 1ml CB - X™ Assay Dye into each tube and mix well .
Incubate for 5 minutes at room temperature .
Read the absorbance at 595nm .
Prepare a standard calibration plot for the determination of protein concentration of the unknown samples .
