Super simple In vivo Hoechst staining of unicellular protists
Add 1 / 1000 Hoechst dye ( 1 uL into 1 mL volume of cells ) . Keep cells with dye protected from light .
Incubate at room temperature for 10 minutes . Keep cells with dye protected from light .
Centrifuge cells at 1000g for 5 minutes .
Resuspend with fresh growing medium .
Use minimum volume of medium in order to plate in to the slide immediately .
Mount your slide and ready for observation under microscope .
