Generating CB - X™ Tables and standard curves for CB - X Protein Assay Optimization .
Prepare duplicate standards of choice in your buffer of choice at the following concentrations : 0 , 0.2 , 0.4 , 0.6 , 0.8 , 1.0µg / µl Transfer 50µl protein standard to 1.5ml centrifuge tubes .
Prepare a standard calibration plot for the determination of protein concentration of the unknown samples .
Use the line equation to generate your own CB - X™ Tables .
This CB - X™ Table will allow all your future protein estimations to be performed without using protein standards , allowing you to carry out rapid , single protein estimations .
Add 1ml pre - chilled ( - 20°C ) CB - X™ and vortex to mix .
Centrifuge at 16,000xg for 5 minutes and carefully remove all the supernatant without disturbing the protein pellet .
Add 50µl CB - X™ Solubilization Buffer - I and 50µl CB - X™ Solubilization Buffer - II to the tube and vortex to dissolve the protein pellet .
Invert the CB - X™ Assay Dye 2 - 3 times to mix and add 1ml CB - X™ Assay Dye to the tube and vortex briefly .
Incubate for 5 minutes at room temperature .
Read the absorbance at 595nm against deionized water using either a 1cm path length cuvette or transfer 200µl assay solution to a microtiter well .
