Adsorbing Viruses on TEM Grids
On the same day as grid prep , use glow discharge to render grids hydrophylic .
Holding the grid in the tweezers , place 5 µl of viral lysate onto the shiny side of the grid ( Figure 1 ) .
Let the sample sit for ~ 3 minutes to allow the viruses to adsorb to the grid .
Stain the viruses using the protocol “Positive and Negative Staining of Viruses on TEM Grids” .
