Red Blood Cell Lysis Protocol : Method II .
Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water .
Warm the 1X solution to room temperature prior to use .
Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of whole blood .
Gently vortex each tube immediately after adding the lysing solution .
Incubate at room temperature , protected from light , for 10 - 15 minutes .
Centrifuge 350 x g for 5 minutes .
Aspirate supernatant without disturbing pellet , and resuspend the pellet in the appropriate buffer ( e . g . , BioLegendCell Staining Buffer , Cat . No . 420201 )
