Lysis Buffer ( 10mM Tris - HCl , 2mM EDTA , 1 % SDS )
Add 2.5ml 1M Tris - HCl pH 7.5 to an autoclaved bottle .
Add 1ml 0.5M EDTA .
Add 2.5g SDS .
Adjust total volume to 250ml with MilliQ
