LB Agar Plates
20g of Agar add ( dH2O ) to 500mL .
Swirl to mix with a stir bar for 10 minutes .
Cover the top of the flask with aluminum foil and label with autoclave tape .
Autoclave on the liquid setting for 30 minutes .
After removing the solution from the autoclave , allow the agar solution to cool to 55°C .
Add the appropriate amount of desired antibiotic to the solution and swirl to mix .
iGEM standard concentrations :
TetKanCam15ug / mL50ug / mL35ug / mL .
Pour ~ 20mL of LB agar per 10cm polystyrene Petri dish .
Place the lids on the plates and allow them to cool for 30 - 60 minutes ( until solidified ) .
Invert the plates and let sit for several more hours or overnight .
Label the bottom of plates with antibiotic and date and store in plastic bags or sealed with parafilm at 4°C .
