Plasmid Miniprep
Pellet culture in a 1.8mL centrifuge tube at 8,000rpm for 1 minute .
Decant Media .
Add 250uL of Resuspension Solution and vortex .
Add 250uL of lysis solution and invert 4 - 6 times .
Add 350uL of Neutralization Solution and invert the tube 4 - 6 times .
Bind the DNA to the column by decanting .
Centrifuge at 13,300 rpm for 1 minute .
Wash the column with 500uL of wash solution and centrifuge for 1 minute .
Discard the flow - thru and centrifuge for 1 minute .
Elute the purified DNA with 50uL of Elution Buffer .
Let incubate for two minutes , then centrifuge for 2 minutes .
Collect the flow - thru in a new centrifuge tube .
