Induction of Chloramphenicol acetyltransferase recombinant protein
Inoculate E . coli BL21 strain containing the recombinant protein of chloramphenicol acetyltransferase in 5mL LB liquid medium ( containing 100ug / mL ampicillin ) , 37 ℃ shaking and culture overnight .
Transfer 1mL overnight cultures in 100 mL ( containing 100ug / mL ampicillin ) LB liquid medium ; 37 ℃ shake culture until OD600 = 0.6 - 0.8 .
Take 10ul samples for SDS - PAGE analysis .
Add IPTG to a final concentration of 0.5 mmol / l , culture 1 - 3h at 37 ℃ .
12,000rpm centrifugation for 10 min , discard the supernatant ; store cell precipitate at - 20 ℃ or - 70 ℃ .
