Yeast DAPI Staining
Grow up yeast in liquid medium overnight .
Add 333 μL ( or 1 volume ) yeast culture to a 1.5 mL microcentrifuge tube .
Add 666 μL ( or 2 volume ) of 100 % Ethanol to the 1.5 mL microcentrifuge tube .
Let the yeast ethanol mixture sit at room temperature for 30 - 60 minutes .
Spin down yeast cells for 1 minute at 2500 RPM .
Pour out the supernatant and resuspend the pellet in 1mL of 1 x PBS , then centrifuge for 1 minute at 2500 RPM .
Pour out the supernatant and resuspend the pellet in 200 μL of a 1 x PBS / 1 : 2000 Dilution DAPI mixture .
Add one drop of the yeast suspended in the PBS / DAPI mixture onto a microscope slide , add a coverslip on top , and go observe the stained yeast .
