Monitoring fluorescence during cyanobacterial physiology / lysis experiments
Advance Preparation : Aliquot 180 µl seawater medium ( e . g . ASW ) into each well of a 96 - well plate .
Keep a log of your plate layout ( which samples in which wells ) .
Plate - Setup : Add 20 µl of sample into the ASW for a 10X dilution .
Mix well with a pipette .
Read fluorescence using the Tecan Infinite Pro 200M plate reader .
Set up a program using the following wavelengths : excitation 440 nm , emission 680 nm for chlorophyll a ; excitation 544 nm , emission 577 nm for phycoerythrin .
Plot fluorescence vs . time to track the real - time progression of cell growth and / or phage infection .
