Standard RNA Synthesis ( E2050 )
Thaw the necessary kit components .
Mix and pulse - spin in microfuge to collect solutions to the bottoms of tubes .
Keep on ice .
Assemble the reaction at room temperature in the following order .
Mix thoroughly and pulse - spin in a microfuge .
Incubate at 37°C for 2 hours .
Optional step : DNase treatment to remove DNA template .
To remove template DNA , add 30 μl nuclease - free water to each 20 μl reaction , followed by 2 μl of DNase I ( RNase - free ) , mix and incubate for 15 minutes at 37°C .
Proceed with purification of synthesized RNA or analysis of transcription products by gel electrophoresis .
