Preparation of NC64A “Ghosts”
Centrifuge the NC64A chlorella in the Sorvall centrifuge at 5,000 rpm , 5 min , 4°C if fresh cells are used .
If frozen cells are used , thaw the pellets and suspend with 50 mM Tris - HCl , pH 7.8 .
Centrifuge the cells in the Sorvall SS34 rotor or HB - 4 rotor at 5,000 rpm , 5 min , 4°C .
Decant the supernatants .
Centrifuge the cells in the Sorvall SS34 rotor or HB - 4 rotor at 5,000 rpm , 5 min , 4°C .
Repeat the 70 % methanol washes 5 - 6 times or until the desired amount of pigment has been removed from the cells .
Store the chlorella “ghosts” at 4°C in 70 % methanol .
Add 10 - 15 mL of 70 % methanol to the chlorella pellets and resuspend the cells completely .
Decant the supernatants .
