Marine Purity Broth
Autoclave BD Difco AC Broth and NaCl Qs to 1 L with distilled water for 20 min .
After broth has cooled , add 16 ml 2 M MgSO4 and 10 ml 1 M CaCl2 .
Dispense 2.0 or 4.5 ml per sterile tube .
Inoculate sterile MPB with 1 : 5 ( vol : vol ) dilution of culture .
Incubate at 22°C
