MBBM Media
Prepare 10 stock solutions per 1 liter Milli - Q H2O each : 25.0 g , NaNO3 ; 2.5 g , CaCl2 * 2H2O ; 7.5 g , MgSO4 * 7H2O ; 7.5 g , K2HPO4 ; 17.5 g , KH2PO4 ; 2.5 g , NaCl ; 50.0 g , disodium EDTA ; 31.0 g , KOH ; 4.98 g , FeSO4 * 7H2O ; per 1 liter acidified H2O ( acificied H2O : 999.0 mL , Milli - Q H2O ; and 1.0 mL , concentrated H2SO4 ) 11.42 g , H3BO3 ; 8.82 g , ZnSO4 * 7H2O ; 1.44 g , MnCl2 * 4H2O ; 0.71 g , MoO3 ; 1.57 g , CuSO4 * 5H2O ; and 0.49 g , CoNO3 * 6H2O .
Add to 950 mL , Milli - Q H2O : 1.0 g , bacto - peptone ; 5.0 g , sucrose ; 10.0 mL of stock solution 1 - 6 ; 1.0 mL of stock solutions 7 - 9 ; 2.0 mL stock solution 10 .
For MBBM agar plates , add 1.5 % agar prior to autoclaving .
For MBBM soft agar , add 0.75 % agar prior to autoclaving .
Autoclave at 121°C for 20 min .
Let media cool to room temperature and add antibiotics : * 700 µg / mL , Ampicillin ; and / or * 10 µg / mL , Tetracycline .
