Double SPRI for Second Generation Sequencing
Add 65 µl SPRI Ampure beads to 50µl of sample .
Mix and incubate for 20 minutes .
Separate on magnet ( 6 min . )
Transfer all supernatant ( about 115 µl ) into a new well ( off magnet ) .
Add 100 µl of SPRI beads .
Mix and incubate for 15 minutes .
Separate on magnet ( 6min ) , discard supernatant .
Wash beads with 60 µl of 70 % EtOH .
Move well off magnet .
Dry beads of EtOH ( ~ 10min ) .
Add 40 µl EB .
Mix and incubate for 3 minutes .
Separate on magnet and transfer eluted product into destination plate
