BetaMark™ x - 40 ELISA Kit ( Chemiluminescent ) Protocol
Label a 1L bottle as “1X Wash Buffer” .
Dilute 5X Wash Buffer 1 : 5 using lab grade water * and mix well .
* Note : Lab grade filtered water such as injection grade , cell culture grade , Reverse Osmosis De - Ionization ( RODI ) .
Label an appropriate sized bottle as “1X Reagent Diluent” .
Label ( 2 ) 1.5mL microcentrifuge tubes as intermediate # 1 & 2 ( use enclosed tubes ) .
Add 990uL of standard diluent to intermediate tubes # 1 & 2 .
Reconstitute 20ug vial of 1 - 40 standard with 80uL of Standard Diluent .
Mix well by inversion , donot vortex .
Concentration will be 250ug / mL .
* Once reconstituted , standard must be used within the same day .
Add 10uL from the vial of reconstituted 1 - 40 standard to 990uL of standard diluent in intermediatetube # 1 .
Mix well by inversion , do not vortex .
Remove 10uL from intermediate # 1 tube and add to 990uL standard diluent in intermediate # 2 tube .
Mix well by inversion , do not vortex .
The final concentration of intermediate tube # 2 will be 25ng / mL .
Label a 50mL centrifuge tube as “1X Incubation Buffer” .
Dilute 2X incubation buffer to 1X by adding 10mL of 2X incubation buffer to 10mL of lab grade water * in the 50mL tube labeled “1X Incubation Buffer” .
Mix well by vortexing .
This will be diluent for the standard curve and samples .
