Lysis Buffer (10mM Tris-HCl, 2mM EDTA, 1% SDS)
Add 2.5ml 1M Tris-HCl pH 7.5 to an autoclaved bottle. 
Add 1ml 0.5M EDTA. 
Add 2.5g SDS. 
Adjust total volume to 250ml with MilliQ
