Transform Stratagene's XL-10 Gold Ultracompetent cells (simplified)
Pre-chill 14mL sterile culture tubes on ice. 
Pre-heat 0.9mL of LB broth to 42°C. 
Thaw XL-10 Gold Ultracompetent cells on ice then mix gently after completely thawed. 
Aliquot cells into pre-chilled sterile culture tubes. 
Swirl tube gently then incubate on ice for 10 minutes swirling gently every 2 minutes. 
Add 0.1-50ng of DNA or 2uL of ligation product. 
Swirl gently then incubate on ice for 30 minutes. 
Heat pulse tube at 42°C for exactly 30 seconds.
The duration of the heat pulse is critical.
Incubate cells on ice for 2 minutes. 
Add 0.9mL of pre-heated LB to each tube. 
Incubate at 37°C for 1 hour with shaking at 225-250 rpm. 
Plate no more than 200uL of transformation mixture per LB-agar plate with antibiotic selection. 
Incubate plates at 37°C overnight.
