Ligation Protocol for NEB PCR Cloning Kit (E1202)
Mix the first 3 components of the reaction.
Add Cloning Master Mix (2X), 5 μl, to a total of 10 μl per ligation reaction.
Incubate at room temperature (25°C) for 5–15 minutes.
Incubate on ice for 2 minutes.
Transform immediately into NEB 10-beta Competent E. coli or store at -20°C.
