QuikChange Mutagenesis
Prepare PCR mixture.
Perform the PCR.
After PCR, add 1ul of DpnI to each pcr tube.
Incubate 1 hour-O/N at 37C.
PCR Purify using Qaigen kit.
Add 5ul of PCR reaction into 25ul DH5alpha or TG1 cells.
Incubate on ice for 5 minutes.
Heatshock at 42C for 45 seconds.
Recover on ice for 2 minutes.
Add 200ul LB (if in 96 well or 8 strip format) to each reaction.
Shake at 37C for 1 hour.
Plate on warmed antibiotic plates.
