Cultivation / Spore Production for Alternaria Solani
Inoculate Mycelium with spores from the stock plate of fresh SNA plates.
Make sure to inoculate only a small amount right in the middle of the plate to allow even and equal spread and growth throughout the plate.
Store plate in a growth chamber with 12/12 h darkness/blacklight. 
Optimal temperature is 22°C. 
Optimal humidity 60-70%. 
Allow to grow for 3 weeks.
Harvest spores adding some 0.0-0.5% tween solution to the plate and scraping gently with a coverslip.
Collect all liquid with spores Filter through four layers of mesh (e.g. cheese cloth) to remove mycelial debris.
